RFP Expressing C57BL/6 Mouse Primary Lung Microvascular Endothelial Cells Size:Frozen Vial (1 x 10^6 cells)Description RFP Expressing Mouse Primary Lung Microvascular Endothelial Cells from Cell Biologics are isolated from the lung tissues of pathogen free laboratory C57BL 6 mice and grown in gelatin pre coated tissue culture flask with Cell Biologics Complete Growth Medium. Cells at passage 3 are harvested from flasks and cryo preserved in vials. Each vial contains 0. 5x106 cells per ml and is delivered frozen. RFP Expressing Mouse Primary Lung
Calibration standards and HRP-labeled detection antibody are added simultaneously to the coated wells
It can be used with the CKS-1301 CryoKING scanner to manage samples
Swissprot No P39023 Gene Accession BC002408 WB Predicted band size 46 kDa WB Positive control WB Recommended dilution 500-2000 IHC predicted cell location Predicted cell location: Cytoplasm or Nucleus IHC positive control Positive control: Human thyroid cancer IHC Recommed dilution Recommended dilution: 30-150 Storage
Immunohistochemical analysis of paraffin-embedded Human thyroid cancer tissue or NECTIN4 antigen-treated (Neutralization experiment) using P101030(NECTIN4 Antibody) at dilution 1/70
and the cells can be digested when they are 80%-90% confluent
MK-6047GFP
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue or DUSP8 antigen-treated (Neutralization experiment) using P102162(DUSP8 Antibody) at dilution 1/50
Adherent cells or suspended cells in logarithmic growth stage were collected in centrifuge tubes and counted
Acts upstream of or within several processes
The encoded protein is a type II cadherin and may play a role in kidney development as well as endometrium and placenta formation
Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin
Cells can be expanded 3-6 passages by the ratio of 1:2 under the cell culture conditions specified by Cell Biologics